Do You Know the Different Types of HPLC Columns?
It is through chemistry processes that several things are produced. The discipline mostly deals with chemicals. There are various Chemistry courses provided at various institutions of higher learning.
Elements of a different nature can be separated in chemical labs. HPLC is a method used in chemistry to separate various elements of a varying nature in a particular mixture. In HPLC, chromatography principles are applied to separate. When the HPLC is separating elements, a mobile phase will flow through a stationary so that a certain component is identified and flows with the mobile phase. The commonly used stationary material is the silica gel because of its nature. You will then need a computer to analyze your data. It is possible to separate components in a mixture because they have different properties.
A HPLC column has several useful parts. The reservoir is vital in storing the mobile phase which is then pumped into the column when required. Time is required for a certain to flow through the column, be separated, and be detected until it is displayed. UV light is the widely used method in component detection.
Various HPLC columns of different nature are applied in chemistry analysis. The type of high performance liquid chromatography column you use will depend on the nature of the elements you are separating. You will find different types of chromatography columns. The difference in polarity of the solvent and the stationary phase makes it possible for compounds to separate in the normal phase chromatography columns.
Reverse phase chromatography columns usually have a less polar stationary phase and a mobile phase that is more polar. There are various types of non-polar stationary phases is use such as C8 and C18 bonded hydrocarbons. The more polar organic components used as solvents in chromatography separation are aqueous organic solutions.
The other common type of HPLC columns is the ion exchange HPLC column. You will use basic or acidic stationary components in ion exchange columns. Solvent material is supposed to be the salt solution. The ionic exchange that happens leads to the separation, identification, and quantifying of various compounds being separated.
In the size exclusion HPLC column, there is a porous stationary phase which makes it possible to separate certain elements depending on their size. You are supposed to use a mixture of polymers to get a porous stationary material.
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